Fig. 2

Transcriptional gene regulation of hPSC-CM differentiation and maturation. A and B Comparison of the differentially expressed gene targets of cardiac TFs which were predicted by using three datasets of hESC/hiPSC-CMs and the significantly associated functions at the early stage (A) and late stage (B). C and D Cardiac TF-gene regulatory networks at the early (C) and late stages (D). Oval nodes represent the target genes of TF logics and are involved in heart development and function (in light red), contraction (in orange), ion transport and handling (in yellow) and stem cell pluripotency regulators (in light blue). Oval nodes with red and green borders indicate up- and down-regulated genes, respectively. The arrow edges indicate regulatory relationships of TF logics and target genes, and the thick edges indicate that the regulatory relations between TFs and target genes are conserved in both hESC-CMs and hiPSC-CMs