Fig. 5

hMSGSCs cultured in a Matrigel 3D system. a Sphere formation of hMSGSCs cultured in a Matrigel 3D system. Bright field microscopy image of self-organized hMSGSCs in Matrigel. Representative image at four different culture time (day 0 [day before start of culture], 2, 6, and 12). Scale bar, 200 μm (left) and 50μm (right). b Gene expression of hMSGSCs cultured in a 3D system. Quantitative real-time PCR analysis of the relative mRNA expression of various markers (Ki67, LGR5, KRT5, KRT14, KRT19, KRT15, AQP5, MUC5B, AMY1B) at different times (day 5, 10, 15, and 20) (n ≥ 3). *P < 0.05, **P < 0.01