Fig. 2
From: Efficient delivery of mesenchymal stem/stromal cells to injured liver by surface PEGylation
Characteristics of PEG-MSCs. A Cell viability of C3H10T1/2 cells after PEG modification. Streptavidin-modified C3H10T1/2 cells were incubated with PEG-biotin, and the cell viability was evaluated by trypan blue exclusion assay. The error bars represent ± SD, n = 4 (ns; not significant). B Proliferation of PEG-C3H10T1/2 cells. The number of PEG-C3H10T1/2 cells or unmodified C3H10T1/2 cells was measured daily by trypan blue exclusion assay. The error bars represent ± SD, n = 4 (ns; not significant). C Differentiation of PEG-C3H10T1/2 cells. Typical images of PEG-C3H10T1/2 cells and unmodified C3H10T1/2 cells differentiated into adipocytes, osteoblasts, or chondrocytes stained with oil red O, alizarin red S, and alcian blue, respectively (scale bars: 150 μm)