Fig. 3
From: Efficient delivery of mesenchymal stem/stromal cells to injured liver by surface PEGylation
Inhibition of cell adhesion by PEG modification. A Number of C3H10T1/2 cells adhering to a culture plate. Unmodified or PEG-C3H10T1/2/Nluc cells were seeded onto a culture plate, and nonadherent cells were removed by PBS wash. The adhering cells were evaluated by measuring the luciferase activity. The error bars represent ± SD, n = 4, *p < 0.05 versus unmodified group, #p < 0.05 versus PEG2k group. B, C Adhesion of C3H10T1/2 cells to monolayered MAECs. Unmodified or PEG-C3H10T1/2 cells were seeded onto monolayered MAECs, and nonadherent C3H10T1/2 cells were removed by PBS wash. The adhering cells were evaluated by measuring the luciferase activity of C3H10T1/2/Nluc cells (B). Two hours after seeding, adhering C3H10T1/2/GFP cells were observed using a fluorescence microscope (C). The error bars represent ± SD, n = 4, *p < 0.05 versus unmodified group, #p < 0.05 versus PEG2k group (scale bars: 100 μm). D, E Immunofluorescence staining for p-FAK in C3H10T1/2/GFP cells. Unmodified or PEG-C3H10T1/2/GFP cells were seeded onto monolayered MAECs. Two hours after incubation, cells were stained and observed using a confocal laser scanning microscope (D). Furthermore, the fluorescence intensity from p-FAK was measured by ROI analysis (E). The error bars represent ± SD, n = 3, 15–20 cells analyzed per sample, *p < 0.05 versus unmodified group (scale bars: 20 μm)