Fig. 2

Mitochondrial morpho-functional analysis and evaluation of OxPhos complexes expression during OB differentiation of hDPSCs. A Representative laser scanning confocal microscopy imaging (LSCM) of mtΔΨ by the fluorescent probe TMRE in hDPSCs during OB differentiation (right panels); digital magnification of details is shown at the bottom of the pictures after false colour rendering and thresholding to remove background (ImageJ software). The histogram on the right shows the quantitative analysis of the TMRE-pixel density/cell; values are means ± SEM of three independent experiments under each condition where the digitalized fluorescence images from at least five randomly selected optical fields (each containing about 10–20 cells) were analysed by ImageJ tools; *P < 0.05. B Representative cropped immunoblotting of OxPhos complexes protein expression during OB differentiation and normalized densitometric analysis (right histogram) performed for three independent blots; Full-length blots are presented in Additional file 1: Fig. S2. β-actin was used as loading control; *P < 0.05; #P < 0.01; §P < 0.001 versus undifferentiated cells