Fig. 4

Evaluation of ROS content in hDPSCs undergoing OB differentiation. Representative LSCM imaging of ROS production during OB differentiation of hDPSCs assessed by DCF-DA (A) and MitoSox (B) probes, respectively. Where displayed enlarged details of the optical field (white square) rendered in false colours are shown at the bottom of the respective panel. The upper and lower sequences of images in (A) and (B) refer to differentiation in the absence and in the presence of the antioxidant Trolox (500 µM, present since the start of the OB induction), respectively. The images are representative of three different preparations yielding similar results. The histograms on the right of panels A and B show quantification of the fluorescence/cell in control and Trolox-treated cells, as elaborated by ImageJ; the bar values are means ± SEM of at least ten different optical fields, each containing 30–50 cells, from 3 independent experiments; *P < 0.05; **P < 0.001; versus untreated undifferentiated samples