Fig. 8

Effect of delayed Trolox treatment on OB differentiation potential of hDPSCs. A Schematic illustration of the experimental design. Trolox treatment of OB-differentiating hDPSCs started at days 0, 7 and 11 after administration of the inducers; the following analyses were carried out at day 21. B Representative photographs of ALP staining of untreated and Trolox-treated hDPSCs as illustrated in (A), and histograms (C) with densiometric analysis of ALP staining (grey bars) and Alp gene expression (yellow bars) performed for three independent experiments; #P < 0.05; ##P < 0.001; versus undifferentiated control; **P < 0.001 versus respective untreated. D Representative immunoblotting for protein expression and phosphorylation of ERK1/2 in untreated and Trolox-treated hDPSCs as illustrated in (A); full-length blots are presented in Additional file 1: Fig. S8. Right panel: densitometric analysis displaying phospho-ERK1/2 (Thr202/Tyr204) / Erk1/2 ratio (normalized to β-actin), expressed as means ± SEM of three independent experiments; **P < 0.001