Table 1 Features of the primers used for real‐time polymerase chain reaction
Gene | Organism | Tann (°C) | Length (bp) | NCBI ref seq or sequence |
|---|---|---|---|---|
Nanog | Homo sapiens | 55 | 90 | NM_024865 |
Myc | Homo sapiens | 60 | 129 | NM_002467 |
Lin28 | Homo sapiens | 55 | 126 | NM_024674 |
Sox2 | Homo sapiens | 60 | 64 | NM_003106 |
Alp | Homo sapiens | 55 | 137 | Forward: GACCTCCTCGGAAGACACTC Reverse: TGAAGGGCTTCTTGTCTGTG NM_000478.4 |
Dspp | Homo sapiens | 55 | 136 | Forward: ATATTGAGGGCTGGAATGGGGA Reverse: TTTGTGGCTCCAGCATTGTCA NM_014208.3 |
Gapdh | Homo sapiens | 60 | 135 | Forward: AGGCTGAGAACGGGAAGC Reverse: CCATGGTGGTGAAGACG |