Fig. 3

hESC-exo alleviate fibrosis-related destruction of lung structure and function. A-B. Comparison of lung weights (A) and BALF total protein concentrations (B) in the three groups of mice. C. Determination of HYP content in BALF from the three groups of mice. D. Representative images of lung sections stained with hematoxylin–eosin (H&E), Masson’s trichrome and Sirius red, and type I collagen- and fibronectin (Fn)-specific antibodies. E. Relative quantification of the collagenous fiber area indicated by Masson’s trichrome staining in lungs. F–I. Western blot analysis of α-SMA (G), collagen I (H), and Fn (I) expression in lung tissue lysates. GAPDH was used as a loading control. The blots of α-SMA, Collagen I, Fn and GAPDH were all cropped (F) and full-length blots were presented in Additional file 8: Fig. S8. n = 3–4; mean ± se; *P < 0.05, **P < 0.01, and ***P < 0.001