Fig. 4

hESC-exo affect the gene expression profile in BLM-induced lungs. A. Venn diagram of DEGs identified from comparison of the non-hESC-exo-treated BLM (BLM + PBS) group versus the control group, the hESC-exo-treated BLM (BLM + exo) group vs. the BLM + PBS group, and the BLM + exo group vs. the control group. B. Venn diagram of DEGs identified as upregulated in the BLM + PBS group compared to those in the control group, and downregulated in the BLM + exo group in comparison to those in the BLM + PBS group. C. Venn diagram of DEGs identified as downregulated in the BLM + PBS group compared to those in the control group, and upregulated in the BLM + exo group in comparison to those in the BLM + PBS group. D, E GO enrichment and KEGG analysis of the 29 DEGs overlapped of upregulated genes in the BLM + PBS group versus NS + PBS group and downregulated genes in the BLM + exo group versus BLM + PBS group and 14 DEGs overlapped of downregulated genes in the BLM + PBS group vs NS + PBS group and upregulated genes in the BLM + exo group vs BLM + PBS group, totally 43 genes. F. Heat map showing the expression profiles of above 43 DEGs. G. To verify the RNA sequencing results, mRNA levels were measured by qPCR. H. qPCR analysis to verify the expression profiles of the top 20 microRNAs expressed in hESC-exo in BLM-induced mice. I. Venn diagram of target genes of miR-17-5p screened by TargetScan, miRbase, and TargetMiner overlapped with the 43 DEGs. And only the target genes of miR-17-5p had overlap with DEGs that was Thbs2. J. Atlas of interaction nodes between miR-17-5p and Thbs2. n = 3–5; mean ± se; *P < 0.05, **P < 0.01, and ***P < 0.001