Fig. 2

hBM-MSCs viability and morphology in substrates with different levels of wettability. A Representative immunofluorescence images of hBM-MSCs cultured for 5 days in reduced serum (2% FBS) on the substrates with levels of wettability and tissue culture plastic (TCP). The cytoskeleton of the cells was stained with actin red (red) and the nuclei with Hoechst (blue). Scale bar = 100 µm. B Elongation of four different hBM-MSCs donors cultured in the topographic substrates. C PrestoBlue viability assay of four different hBM-MSCs donors quantified as relative fluorescence units per cell, relative to the control (TCP). Error bars depict the mean ± SEM of 3 independent experiments with n = 3–4 samples. Asterisk (*) represent p-values < 0.05, (**) p-values < 0.01, (***) p-values < 0.001 and (****) p-values < 0.0001