Fig. 6

Immunosuppressive potency of hBM-MSCs cultured in topographical substrates. A–D Cytokine secretion of hBM-MSCs donors 182 and 271 cultured on topographical substrates. Cells were cultured for 5 days in reduced serum conditions (2% FBS) and quantification of target proteins was performed using enzyme-linked immunosorbent assays (ELISA), except for IDO C–D which was quantified via the kynurenine colorimetric assay and results are displayed as a fold relative to TCP. E Quantification of hBM-MSCs potency of donor 182 to suppress the proliferation of peripheral blood mononuclear cells (PBMCs); immune potency assay (IPA). Cells from donor 182 were seeded on the topographical substrates and co-cultured with PBMCs for 4 days in reduced serum conditions at a 1:0.5 ratio (PBMC:MSC). Proliferation of CFSE-stained PBMCs was measured using flow cytometry. F Evaluation of hBM-MSCs potency to suppress PBMCs proliferation after being cultured for 2 passages in the topographical substrates and then transferred to TCP surface and co-cultured with PBMCs as described above. Error bars depict the mean ± SEM of 2 independent experiments with n = 2 samples. Asterisk (*) represent p-values < 0.05, (**) p-values < 0.01, (***) p-values < 0.001 and (****) p-values < 0.0001