Fig. 4

IL-1β in the serum of ACLF patients can increase the level of IL-8 in hUC-MSCs. A Effects of TNF -α, IL-1β, LPS and IFN-γ on the expression of IL-8 in hUC-MSCs. B The expression levels of IL-1β and TNF-α in the serum of different groups were detected by ELISA. C After IL-1β stimulation of hUC-MSCs for 24 h, changes in the pathways related to IL-8 expression in hUC-MSCs were detected by Q-PCR. D The effect of IL-1β on the NF-κB signaling pathway and IL-8 expression in hUC-MSCs was detected by Western blotting. Full-length blots are presented in supplementary figure s2. *P < 0.05; **P < 0.01; ***P < 0.001 (DPI:300)