Fig. 2

scRNA-seq analysis of PP-stage differentiating cells reveals emergence of extraembryonic cell populations in the hydrogels. a UMAP of scRNA-seq after 4-stage PP differentiation in 2D and 3D (0.5 kPa GelNB hydrogel) conditions. Data represent 7468 and 8447 total cells for 2D and 3D conditions, respectively. b Percentage of cell populations from scRNA-seq analysis in 2D and 3D conditions. c–f Dot plots of upregulated genes in pancreatic (c), ductal (d), foregut (e), and extraembryonic (f) clusters