Fig. 6

Small molecule inhibition of BMP and Wnt signaling pathways during pancreatic differentiation. a Representative live/dead and immunostained images of treated cells at the PP stage. Quantification of b NKX6.1 and c PDX1 fluorescence normalized to the nuclear stain in the immunostaining images. Statistical significance was determined by a one-way ANOVA relative to the vehicle control. (**p < 0.01, N = 3 images per condition). d PDX1/NKX6.1/SOX9/PTF1A mRNA expression with or without LDN treatment starting at the S2 stage. e PDX1/NKX6.1/SOX9/PTF1A mRNA expression with or without LDN/XAV treatment starting at the S3 stage. For panels d and e, significance was determined via two-way ANOVA relative to the vehicle control. (**** represents p < 0.0001, N = 3 samples per condition)