Table 2 Primer sequences used for RT-qPCR experiments
Gene symbol | Gene name | Sequence forward | Sequence reverse |
|---|---|---|---|
ADAMTS5 | A Disintegrin and Metalloproteinase with Thrombospondin motifs | CTCCACGCAGCCTT-CACTGT | TGGGTGGCATCGTA-GGTCTG |
AGG | Aggrecan | TCGAGGACAGCGAGGCC | TCGAGGGTGTAGCGT-GTAGAGA |
AP | Alkaline phosphatase | CCACGTCTTCACAT-TTGGTG | GCAGTGAAGGGCTT-CTTGTC |
COL I | Collagen type I | CCTGGATGCCATCA-AAGTCT | CGCCATACTCGAAC-TGGAAT |
COL IIB | Collagen type IIB | CAGACGCTGGTGCTGCT | TCCTGGTTGCCGGACAT |
COL X | Collagen type X | TGCTGCCACAAATA-CCCTTT | GTGGACCAGGAGTA-CCTTGC |
LINK | Link protein | TTCCACAAGCACAA-ACTTTACACAT | GTGAAACTGAGTTT-TGTATAACCTCTCAGT |
MMP13 | Matrix Metallopeptidase 13 | TAAGGAGCATGGCG-ACTTCT | GTCTGGCGTTTTTG-GATGTT |
RSP9 | Ribosomal small protein 9 | ATGAAGGACGGGAT-GTTCAC | GATTACATCCTGGG-CCTGAA |
RUNX2 | Runt-related transcription factor 2 | CGGAATGCCTCTGC-TGTTAT | TTCCCGAGGTCCAT-CTACTG |
SOX9 | Sex Determining Region Y-Box 9 | AGGTGCTCAAAGGC-TACGAC | GTAATCCGGGTGGT-CCTTCT |
SPARC | Secreted protein, Acidic, Cysteine-Rich | AAAGCACAAGGCAG-AAAGGA | GGTGGGCTTGATGA-CTCTGT |