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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Single-cell sequencing reveals the existence of fetal vascular endothelial stem cell-like cells in mouse liver

Fig. 1

Single-cell analysis of liver ECs across development. a Schematic of experimental design for the single-cell analysis pipeline. Livers from E14, E18, P7, P14, and W8 were dissected and dissociated into single cells. Sorted CD31+CD45− ECs were collected and analyzed using the 10X Genomics Chromium platform. For each time point, one scRNA-seq library was generated using pooled tissues dissected from ± 6 individual animals. b UMAP visualization of ECs from all time points (n = 20.799 cells) colored and labeled based on cell clusters (left) and time point (right). c Dot plot of differentially expressed genes in each cluster. Gene expression in Log10 (TPM)

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Stem Cell Research & Therapy

ISSN: 1757-6512