Fig. 2

CD157⁺CD200⁺ VESCs emerge from CD157⁻CD200⁺ ECs during the perinatal period. a UMAP projection of each EC clusters from E14 to W8. b Feature plot showing relative distribution and expression of CD157/Bst1 (top) and CD200 (bottom) for each time point. Each purple dot represents a single cell. Black arrows: CD200 + ECs; black arrowheads: CD157 + ECs. c Quantification of total ECs among all cells in liver. d FACS analysis of the liver from different time points showing the emergence of CD157⁺CD200⁺ among CD31⁺CD45⁻ ECs during early postnatal period. e, f Quantification of CD157⁺CD200⁺ ECs (e) and CD157⁻CD200⁺ ECs (f) among total ECs. g Immunofluorescence analysis of CD157 and CD200 in portal vein of E18, P7, and P21 liver. White arrowheads show the expression of CD157 within the PV. PV, portal vein. Data are shown as means ± SEM. Statistical analysis using unpaired two-tailed t-test, *** p < 0.0001 and **p < 0.005, ns p > 0.05