Fig. 4

Perinatal CD157⁻CD200⁺ ECs and adult CD157⁺CD200⁺ ECs possess similar endothelial colony-forming ability. a Schematic overview. A total of 1000 liver ECs from fetal, neonatal, and adult liver were cultured on OP9 feeder cells for 10 days. b Cultured ECs from different time points were stained for CD31 (low-power view). The colony is defined as a cluster of > 50 ECs. There are no CD157 + CD200 + ECs at day E18. Experiments were repeated at least three times. c Quantification of the number of EC colonies formed by each fraction of ECs across development. d Schematic overview. Limiting dilution analysis of liver ECs from GFP mice cultured on OP9 feeder cells for 10 days. Experiments were repeated at least three times. e ECFCs frequency in each EC fraction calculated using the online algorithm, n = 3. Data are shown as means ± SEM. Statistical analysis using unpaired two-tailed t-test, ***p < 0.0001, **p < 0.005, and *p < 0.05, ns p > 0.05