Fig. 3

Transcriptomic profiling of HSC-NK cells in response to K562-mbIL21 feeder cell stimulation. A Schematic illustrating the RNA sequencing analysis of day 0 and day 14 HSC-NK cells. B Volcano plot comparing day 0 HSC-NK cells in a 330 mM osmotic environment to the 300 mM control. C Volcano plot comparing day 14 HSC-NK cells in a 330 mM osmotic environment to the 300 mM control. D Volcano plot comparing day 14 HSC-NK cells to day 0 HSC-NK cells. The data for each group comprises integrated data from the 300 mM and 330 mM conditions. E Gene Ontology (GO) analysis of significantly enriched genes in expanded day 14 HSC-NK cells, as depicted in (D). Bar charts illustrate the top 20 significantly enriched GO terms for molecular function, biological process, and cellular components in day 14 HSC-NK cells