Fig. 4

Anti-tumor activity of HSC-NK cells compared to PB-NK cells. A Schematic of the in vitro analysis of NK cell anti-tumor abilities. Tumor cells were transduced with a lentiviral vector to express GFP protein. After co-culture of NK cells and tumor cells, the residual tumor cells were analyzed by flow cytometry. B–D Flow cytometry-evaluated killing activity against HepG2 (B), MOLM-13 (C), and SKOV-3 (D) after a 24-h co-culture of NK cells and target cancer cells at the indicated E:T ratios. Data in B, C, and D are displayed as mean ± SEM from 3 independent experimental replicates. A two-way ANOVA test was performed, and the results are presented in Additional file 1: Fig. S3