Fig. 3

Altered lipid metabolism in the SVZ of adult mice. A GO analysis of genes in SVZ of Seipin KO and WT mice. B Pie charts showing the percentage of lipid metabolism in SVZ primary metabolism of adult and juvenile Seipin KO mice. C KEGG pathway analysis showing the lipid metabolism-related DEGs, including glycerophospholipid, phospholipase D, and sphingolipid signaling pathways between adult Seipin KO and WT mice. D Pattern diagram of SVZ region by MALDI-TOF MS analysis. CX: cortex, CPu: caudate putamen, CC: corpus callosum, LV: lateral ventricle. E: MALDI-TOF MS showing significantly accumulated lipids in the SVZ of adult Seipin KO mice and their representative m/z ratio peaks. Scale bar = 100 μm. F Heatmap analysis showing the most significantly accumulated lipid components in the SVZ of adult Seipin KO mice (n = 3). G: Oil Red O detection of LD in the SVZ of the adult brain of Seipin KO mice (n = 6), Scale bar = 50 μm. H Pearson correlations analyses between neurogenesis impairment and the area of LD accumulation (n = 6). Data were shown as means ± SD. *p < 0.05 vs. adult WT