Fig. 6

AT transplantation impacting the lipid metabolism and activity of NSCs via the DG-PKCα pathway. A Oil Red O staining shows LD in SVZ of Seipin KO and WT mice with or without AT transplantation (n = 5). *p < 0.05 vs. WT-Sham, #p < 0.05 vs. KO-Sham. Scale bar = 50 μm. B and C Imaging mass spectrometry (B) and heatmap (C) analysis of lipid profiles in SVZ of Seipin KO mice with or without AT transplantation to show significantly reduced lipids (n = 3). Scale bar = 100 μm (B). D and E Western blotting to show the protein expression of PKCα in SVZ of Seipin KO mice before and after AT transplantation (D) and in NSCs derived from SVZ treated with 0, 50, 100 μM DiC8 (E). *p < 0.05 vs. WT-Sham, #p < 0.05 vs. KO-Sham, full-length blots are presented in Additional file 3: Fig. S3. F and G Immunofluorescence analysis for BrdU (F) and Tuj1 (G) to show the impact of DiC8 (100 μM) and PKCα inhibitor on proliferation and differentiation of NSCs derived from SVZ. Scale bar = 50 μm. *p < 0.05. Data were shown as means ± SD