Fig. 2

BMSC transplantation promotes encephalo-myo-synangiosis mediated collateral neovascularization in BCAS mice. A, B Representative images of immunofluorescence staining A and quantitative data of the number of blood vessels and astrocytes B in the contact between local temporal muscle and cerebral cortex 30 days after BMSC transplantation: CD31 (red), GFAP (red). The merged image fuses red and blue fluorescent images (9 animals per group). Scale bar = 50 μm. C Representative images of laser speckle imaging of regional cerebral blood flow and the relative blood flow of the surgical area compared with the nonsurgical area. (8 animals per group). D Representative images of 3D vascular structure in the attachment area of temporal muscle by light sheet microscopy