Fig. 5

BMSCs and concentrated BMSC-CM can improve the behavior and angiogenesis of mice with chronic brain ischemia. A The Morris water maze was used to detect the frequency and distance of mice entering the third quadrant, effective zone and escape platform 30 days after EMS and concentrated BMSC-CM application. (6 animals per group). B Representative images of immunofluorescence staining and quantitative data of the number of blood vessels in contact between the local temporal muscle and cerebral cortex 30 days after concentrated BMSC-CM application: CD31 (red) and GFAP (green). The yellow dotted line represents the brain surface. The merged image fuses red, green and blue fluorescent images. (6 animals per group). Scale bar = 50 μm