Fig. 2

ENSCs give rise to glial cells and neurons, including nNOS-expressing subtype, 6 weeks following transplantation to mouse colon in vivo. Transplanted ENSCs were identified as tdT + cells and fibers, with co-localization with the glial marker, Plp1 in the muscularis layer (A) while some projecting fibers derived from transplanted cells are Plp1 negative (A′, a magnified view of dotted box shown in A). Wholemount immunostaining with Tuj1 (B–B′″) shows neuronal differentiation of transplanted ENSCs (B′″, arrow, B′″ is a magnified view of dotted box shown in B). In the mucosal layer (C–C″), transplanted ENSC-derived neuronal fibers are integrated with host neurons (C′″ is magnified view of dotted box shown in C″). A subpopulation of ENSC-derived neurons express nNOS (D–D″″, arrow, D′″ is magnified view of dotted box shown in D). Scale bars 1 mm (A) and 200 µm (B–D)