Fig. 4

Restoration of circular smooth muscle activity in nNOS^−/− (KO) colon by transplantation of ENSC. A Representative traces of mechanical response to EFS in NANC conditions. EFS induced a biphasic response characterized by a relaxation (Phase-I) followed by a rebound contraction (Phase-II). B and C Quantification of EFS-induced relaxation (area under curve) and rebound contraction (maximum effects are shown as absolute change from basal values). D and E Group data illustrate the effects of L-NAME on EFS-induced muscle activity in transplanted mice. Error bars represent mean ± SEM. Numbers of animals are shown in parentheses. ***p < 0.001, **p < 0.01, and *p < 0.05