Fig. 3

Histological analysis of mice lung sections at various timepoints after LPS-induced ALI. Representative histological images are shown for 1 h, 3.5 h, 10 h, 24 h and 72 h (panels A–E, respectively). F Histological lung injury score was highest at 72 h and evaluated as per the American Thoracic Guidelines, which includes assessment for: (1) intra-alveolar cell infiltration, (2) cell infiltration into interstitial space, (3) hyaline membranes, (4) proteinaceous debris in airspace (e.g., fibrin strands) and (5) alveolar septal thickening [30]. N = 3–5 per timepoint. Data are presented as mean ± SEM with a maximum score of 1.0, **P < 0.01. Scale bar represents 50 μm