Fig. 7

Biodistribution of DiR-labeled EVs derived from HEK293T cells (HEK-EVs). A Representative live and ex vivo organ images at 24 h, 48 h and 72 h after HEK-EV administration in ALI mice. Comparison of EV distribution between mice with LPS-induced ALI and healthy controls found significantly elevated B lung accumulation of EVs during ALI with no changes to C liver or D spleen, N = 5–6 per group for ALI mice, N = 3–6 for healthy mice. E Comparison of organ distribution of EVs derived from MSCs and HEK293T cells during ALI, N = 4–10 per group for MSC-EVs, N = 5–6 per group for HEK-EVs. Data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001