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Table 2 Characterization and preparation methods of the MSCs

From: What is the optimal dose of adipose-derived mesenchymal stem cells treatment for knee osteoarthritis? A conventional and network meta-analysis of randomized controlled trials

References

Study

Country

Origin

Characterization standards

Preparation methods

(location, method)

Compliance with GMP standards

[11]

Freitag et al., (1) 2019

Australia

AD-MSCs

Surface marker (s CD90+, CD73+, CD 105+, CD14−, CD19−, CD34−, CD45−)

Onsite laboratory,

Enzymatic digestion

 

[12]

Garza et al., (1) 2020

USA

AD-MSCs

Surface marker (CD45−, CD31−, CD34+)

N.A,

Enzymatic digestion

 

[13]

Koh et al., 2012

South Korea

AD-MSCs

NO

Operating room,

Enzymatic digestion

 

[14]

Lee et al., 2019

South Korea

AD-MSCs

Surface marker (purity: CD31, CD34, CD45; identity: CD73, CD90)

Laboratory,

Enzymatic digestion

 

[15]

Lu et al., 2019

China

AD-MSCs

Surface marker (Cd105+, CD73+, CD90+, CD45−, CD34−, CD14−, CD11b−, CD79a−, CD19−, HLA II−)

Laboratory,

Medium culture

Yes

[16]

Jo et al., 2017

Korea

AD-MSCs

Surface marker (CD31, CD34, CD45; identity: CD73, CD90)

N.A,

N. A

Yes

[17]

Kuah et al., 2018

Australia

AD-MSCs

NO

Medicine Centre,

N. A

Yes

[18]

Chen et al., 2021

China

AD-MSCs

Surface marker(unspecified), tri-lineage differentiation

N.A,

Enzymatic digestion

 

[19]

Bastos et al., 2019

Portugal

BM-MSCs

NO

N.A,

N. A

 

[20]

Lamo‑Espinos et al., 2016

Spain

BM-MSCs

Surface marker (CD90+, CD73+, CD44+, CD34−, CD45−)

N.A,

Medium culture

Yes

[21]

Emadedin et al., 2018

Iran

BM-MSCs

NO

N.A,

N.A

 

[22]

Vega et al., 2015

Spain

BM-MSCs

Surface marker (CD90+, CD73+, CD105+, CD166+, CD34−, CD45−, CD14−, CD19−, HLA-DR-)

Operating room,

enzymatic digestion

 

[23]

Guptaet al., 2016

India

BM-MSCs

Surface marker (CD90+, CD73+, CD105+, CD166+, CD34−, CD45−, CD14−, CD19−, CD133+, HLA-DR-)

N.A,

enzymatic digestion

Yes

[24]

Matas et al., 2019

Chile

UC-MSCs

Surface marker (CD73+, CD90+, CD 105+, CD14−, CD34−, CD45−)

Laboratory,

Medium culture

Yes

[25]

Ha et al., 2018

China

UC-MSCs

Surface marker CD19−, CD34−, CD45−, CD11b−, HLA-ABC-, CD29+, CD44+, CD73+, CD90+, CD105+

Central Laboratory,

Enzymatic digestion

 

[26]

Yang et al., 2017

China

UC-MSCs

NO

Central Laboratory,

Enzymatic digestion

Yes