Fig. 4
Activation of Wnt/β-catenin signaling suppresses cardiac Na + channel Nav1.5 expression and function in iPSC-CMs. A Bar graph to compare the mRNA expression of SCN5A in control iPSC-CMs after treatment with CHIR99021 (1.25, 2.5, 5, 10 and 20 μM) or IWR-1 (1.25, 2.5, 5, 10, 15 and 20 μM) for 48 h, respectively. n = 4–7 technical replicates. ****p < 0.0001. B Western blot analysis of Nav1.5 expression in control iPSC-CMs treated with DMSO only (vehicle), 5 μM CHIR99021 or 10 μM IWR-1 for 72 h, respectively. Full-length blots are presented in Additional file 1: Fig. S8. C Bar graph to compare the Nav1.5 expression between three different groups. n = 3 culture replicates. *p < 0.05 and **p < 0.01. D Representative sodium current tracings recorded from three different groups. E Comparison of IV curve of sodium current between three different groups. F Bar graph to compare peak sodium current density at -20 mV between three different groups. n = 7–11 cells. ***p < 0.001 and ****p < 0.0001. G Comparison of SSA and SSI of sodium current between three different groups. H, I Bar graphs to compare V1/2 of SSA and SSI of sodium current between three different groups. n = 7–14 cells. *p < 0.05