Fig. 6
Inhibition of Wnt/β-catenin rescues Na + channel defects and arrhythmic phenotypes in BrS iPSC-CMs. A. Bar graph to compare the mRNA expression of SCN5A in control iPSC-CMs, BrS iPSC-CMs treated with DMSO only, and BrS iPSC-CMs treated with 10 μM IWR-1. n = 6–8 technical replicates. ***p < 0.001 and ****p < 0.0001. B Western blot analysis of Nav1.5 expression in three different groups. Full-length blots are presented in Additional file 1: Fig. S12. C Bar graph to compare the Nav1.5 expression between three different groups. n = 4 culture replicates. ***p < 0.001. D Representative sodium current tracings recorded from three different groups. E Comparison of IV curve of sodium current between three different groups. F Bar graph to compare peak sodium current density at − 20 mV between three different groups. n = 10–17 cells. ***p < 0.001 and ****p < 0.0001. G Comparison of SSA and SSI of sodium current between three different groups. H, I Bar graphs to compare V1/2 of SSA and SSI of sodium current between three different groups. n = 8–18 cells. *p < 0.05 and **p < 0.01. J Representative action potential tracings recorded by single-cell patch clamp from three different groups. Dashed lines indicate 0 mV. Red arrows indicate putative arrhythmias in BrS iPSC-CMs treated with DMSO. K Bar graph to compare the percentage of iPSC-CMs with arrhythmias between three different groups. n = 20–41 cells