Fig. 3

Phenotypic characterization of murine ASCs from db/db mice in high-glucose (dbHG) or low-glucose (dbLG) conditions. A The expression level of ASC surface markers is shown as the proportion of positively stained cells relative to the isotype control. Both groups of ASCs were negative for the hematopoietic markers CD31 and CD34 but positive for the mesenchymal stem cell-related markers CD90. B Murine ASCs were cultured in adipogenic or osteogenic induction medium, and cells were stained with Oil Red O for the detection of adipogenesis and Alizarin Red for the detection of osteogenesis. Scale bar = 200 μm. C Cumulative population doubling curve showing faster proliferation of the dbLG group. D Quantification of SA-β-gal-positive cells in five randomly selected power fields revealed more senescent cells in the dbHG group. E After cultured in LG and HG conditions, murine ASCs were examined for ROS production. Significantly more ROS production was noted in the dbHG condition. *p < 0.05, **p < 0.01 relative to dbHG. ASC adipose-derived stem cell, SA-β-gal senescence-associated β-galactosidase, ROS reactive oxygen species