Fig. 7

Immunohistochemistry and dbASC retention in the wound tissue. A Representative images of the CD31 immunohistochemical sections of wound explants at day 5 and day 21. Red arrows indicate CD31-expressing cells around small vessels. Quantitative analysis of the CD31-expressing cells in high-power field images was shown. Scale bar = 50 µm. *p < 0.05 relative to PBS. B Representative images of wound sections stained with anti-CD68 and anti-α-SMA at day 21. Scale bar = 50 µm. Quantitative analysis of the immunohistochemical signals of CD68 and α-SMA showed no differences among different groups. C Residual dbASCs in the wound tissues at day 5 were visualized by Qtracker labeling. Scale bar = 100 µm. Significantly more retaining dbASCs were found in the wound tissue treated with dbLG. **p < 0.01 relative to the dbHG group. dbASC murine ASCs from db/db mice, dbHG dbASCs cultured in HG condition, dbLG dbASCs cultured in LG condition