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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Functional and long-lived melanocytes from human pluripotent stem cells with transient ectopic expression of JMJD3

Fig. 3

EMCs transfer pigments to keratinocytes in co-culture. A DOPA (3,4-dihydroxyphenylalanine) reaction of EMCs (upper panels) and TMCs (lower panels). B Immunocytochemical analysis of keratinocytes on EMCs or TMCs with antibodies against CK14 (red) and TRP1 (green). Nuclei were counterstained with DAPI (blue). Lower right inlets show magnified keratinocytes with TRP1-positive inclusion. Green dotted lines show the outline of melanocytes. C, D Flow cytometric analysis of EMCs, TMCs, and keratinocytes with antibodies against CK14 and TRP1. Red and green dots indicate CK14 + /TRP1- keratinocytes and CK14-/TRP1 + melanocytes, respectively. Purple dots indicate double-positive CK14 + /TRP1 + keratinocytes. C Keratinocytes and melanocytes were mixed just before the analysis. D Keratinocytes were co-cultured with EMCs or TMCs for 6 days

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Stem Cell Research & Therapy

ISSN: 1757-6512