Fig. 1
Phenotypic characterization of bone marrow-derived MSCs in this study. A Method for varying low (MSCLC) and high confluency (MSCHC) of BM-MSCs from 6 donors. B Representative images of MSCLC and MSCHC for Donor 1 (scale bar: 1 mm). C Population doubling time of MSCLC and MSCHC for Donor 1 to 6. D Flow cytometry-mediated characterization of surface markers to determine % positive BM-MSCs in each experimental condition for this study. E In vitro differentiation of MSCLC and MSCHC from Donor 1. Alizarin red staining for calcium deposits detected in osteoblasts, Oil red O staining for oil droplets in adipocytes and collagen II staining was performed for chondrocytes. Scale bar: 100 μm. F Differentiation assays performed in technical triplicates and quantified for Donor 1 as compared to undifferentiated cells (UD). One-way ANOVA (Tukey's multiple comparison test) or Mann–Whitney test was performed between selected pairs as statistical test. *p ≤ 0.05; **p ≤ 0.01 *** p ≤ 0.001; ns represents non-significant