Fig. 4
Differential gene expression analysis of MSCHC and MSCLC. A Principle component analysis (PCA) plot show 2 clusters among datasets of MSCHC and MSCLC in 6 donors, with at least 2 technical replicates for each donor. B Volcano plot with top 10 up-regulated and down-regulated differentially expressed genes (DEGs) by comparing MSCHC over MSCLC for all donors measured by RNA-seq analysis. C Unsupervised hierarchical clustering using average linkage with Euclidean distance for DEGs. D Venn diagram showing number of significant DEGs found in respective section. E Significantly enriched signalling pathways (ranked by p-value) associated with 731 DEGs unique in responders (D1, D2, D4, D5). F Proposed model for establishing the relationship between MSC culture confluency and MSC-mediated immunosuppression on T cells. Created with BioRender.com