Fig. 1

Dip2b is dispensable for pluripotency maintenance of mouse ESCs. A Schematic of Dip2b knockout. B Karyotype analysis of KO ESCs after 12 passages. C Representative morphological images of WT and KO clones (n = 3). Scale bar, 25 μm. D qRT-PCR (left) and PCR (right, full-length gels are presented in Additional file 2) of Dip2b expression in WT and Dip2b knockout (n = 3). E qRT-PCR of representative pluripotent genes expression in WT and KO ESCs (n = 3). F Representative immunofluorescence staining of pluripotent marker NANOG and OCT4 for WT and KO ESCs (n = 3). Scale bar, 50 μm. G A450 for CCK8 assay in three time points during culture (n = 3). H Cell cycle analysis using PI staining in WT and KO ESCs. The populations of different phases were calculated in FlowJo (v10.8.1) (n = 3). I Representative morphology of EB formation derived from KO ESC expressing mCherry (n = 3). Scale bar, 25 μm. J qRT-PCR of representative pluripotent and lineage genes expression at day 6 post differentiation in KO ESCs (n = 3). The error bars represented the mean ± SD, and the significance level was calculated by Student’s t test (two-tailed, equal variance) (ns, not statistically significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)