Fig. 1

Notch signaling is activated and required for the generation of human MuRC in vitro. A Total mRNA were isolated from human myoblasts in growth medium (MB), 48 h MuRC and 96 h MuRC. qRT-PCR of Pax7, MyoD and the Notch target gene, Hes1. Results are presented as mean ± SEM.; n = 6, one-way ANOVA. B Total protein extracts were isolated from human myoblasts in growth medium (MB) or after 24 h in DM. MuRC and myotubes fractions were separated after 48 h, 72 h or 96 h in DM. Western blot analysis for Pax7, MyoD and the notch intra-cellular domain (NICD). Results are presented as mean ± SEM for myoblasts (MB), 48 h MuRC and 96 h MuRC; n = 6, one-way ANOVA. Full-length blots are presented in Additional file 1: Fig. S1 C Immunofluorescence staining for Pax7 (green), MyoD (red) and DAPI (blue) of human myogenic culture after 48 h in differentiation medium (DM) in the presence of DMSO or the Notch inhibitor, DAPT. The images shown are representative of the three independent experiments. Results are presented as mean ± SEM; n = 3 with 3 images analyzed per experiment, unpaired t test. Scale bar: 10 μm