Fig. 2

Human MuRC are transcriptionally less active and possess a primary cilium. A Human myoblasts and 48 h-MuRC were stained with Hoescht 33,342 and pyronin Y and analyzed by flow cytometry. One histogram is shown, representative of three independent experiments. B Cell size of human myoblasts and of 48 h-MuRC, measured immediately after trypsinization, mean ± SEM, n = 6, one-way ANOVA. C Immuno-detection of primary cilia on myogenic culture after 48 h in DM and stained with antibodies against acetylated α-tubulin and γ-tubulin. D Immuno-detection of primary cilia on human myogenic cells in different cellular states; proliferating myoblasts (MB) and quiescent MuRC (48 h-MuRC). Staining with antibodies against Pax7 and acetylated α-tubulin (mark cilia) and DAPI. Values shown on the histogram are mean ± SEM; n = 8, unpaired t test