Fig. 3

Human MuRC are heterogeneous for Pax7 expression. A Immunofluorescence staining for Pax7 (green), MyoD (red) and DAPI (blue) of human myogenic cells after 48 h in differentiation conditions (48 h DM). Orange squares identify Pax7^High MuRC and red circles identify Pax7^Low MuRC. Quantification of Pax7^High MuRC and Pax7^Low MuRC after immunofluorescence staining is presented as mean ± SEM; n = 4, unpaired t test. B Flow cytometry analysis of human myoblasts (blue) and of human MuRC isolated after 48 h (light green) or 96 h (dark green) in DM, fixed, permeabilized and stained with antibody against Pax7 and CD56. The isotype control staining for Pax7 is presented in black for all analysis. The histogram represents the percentage of Pax7^High human MuRC after 48 h or 96 h in DM. Results are presented as mean ± SEM; n = 8, unpaired t test