Fig. 4

Transcriptional profiles of human Pax7^High and Pax7^Low MuRC subpopulations. All human cells (myoblasts, MuRC-Pax7^High and MuRC-Pax7^Low) were fixed/permeabilized and stained for Pax7 before flow cytometry and mRNA isolation. A Biological samples were represented by principal component analysis (PCA). PC1/PC2 analysis demonstrated that human MuRC and human myoblasts displayed very different expression profiles (PC1), whereas for each biological sample analyzed by RNA-seq, we observed that Pax7^High and Pax7^Low MuRC are very similar in gene expression profile. B Volcano plot of the supervised analysis comparing myoblasts and Pax7^High MuRC and comparing Pax7^High MuRC and Pax7^Low MuRC transcriptomes. Strong transcript modifications between myoblast and Pax7^High MuRC were observed with 3393 modified genes whereas the changes were moderate between Pax7^High MuRC and Pax7^Low MuRC with 103 modified genes (Log2FC > 0.5, P < 0.01). C Heatmap showing the number of normalized reads of MuRC-RNA-seq data for the 103 modified genes between Pax7^High MuRC and Pax7^Low MuRC populations. (D, E) Heatmap showing the average number of normalized reads of myoblasts (n = 5), of Pax7^High MuRC (n = 6) and of Pax7^Low MuRC (n = 6) RNA-seq data for a selection of genes