Fig. 5

Human MuRC significantly reduced glycolysis, maximal respiration and ATP-linked respiration as compared to myoblasts. ECAR (extracellular acidification rate) and OCR (oxygen consumption rate) were assessed in human myoblasts and in human MuRC isolated after 48 h or 96 h in DM (48 h-MuRC or 96 h-MuRC). A After the evaluation of glycolysis post-glucose injection, oligomycin and 2-deoxy-glucose (DG) were added as shown (n = 4). Glycolysis, maximal glycolytic activity and glycolytic reserve were measured for human myoblasts, 48 h-MuRC and 96 h-MuRC (mean ± SEM, n = 4, one-way ANOVA). B After the evaluation of basal respiration, oligomycin, FCCP (carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone) and ROT/AA (rotenone/antimycin A) were added as shown (n = 4). Basal respiration, ATP-linked respiration and maximal respiration were measured for human myoblasts, 48 h-MuRC and 96 h-MuRC (mean ± SEM, n = 4, one-way ANOVA). Seahorse data were normalized to the number of cells per well