Fig. 6

Human MuRC overexpressed active AMPKα1 in correlation with increased fatty acid uptake. A Western blot analysis of AMPKα1, acetyl-CoA carboxylase (ACC) and phospho-ACC (pACC) in human myoblasts (MB), human 48 h-MuRC and 96 h-MuRC. Full-length blots are presented in Additional file 1: Fig. S2. Quantifications are given in (B) after the normalization of protein level to α-tubulin. Data are presented as mean ± SEM; n = 6, one-way ANOVA. C Representative histogram after flow cytometry analysis of glucose (2-NBDG) and fatty acid (palmitate rhodamine) uptake by human myoblasts (MB), 48 h-MuRC and 96 h-MuRC. D Quantification of the median fluorescence intensity (MFI) ratio for 2-NBDG and palmitate uptake of five independent experiments. Data are presented as mean ± SEM; n = 5, one-way ANOVA