Fig. 5

SARS-CoV-2 genes interact with host proteins in hPSC-CMs and reduce cellular ATP levels. A The scheme showing Co-IP MS method to globally study SARS-CoV-2 protein interactors in hESC-CMs. B–D GO analysis of Nsp6 interactors (B), Nsp8 interactors (C) and M interactors (D). E–G Signaling pathway (SP) analysis of Nsp6 interactors (E), Nsp8 interactors (F) and M interactors (G). H Protein–protein interaction (PPI) shows Nsp6 interactors are associated with cardiac hypertrophy and mitochondrial dysfunction. I Protein–protein interaction (PPI) shows Nsp8 interactors are associated with cardiac hypertrophy, mitochondrial dysfunction and cardiac arrhythmia. J Protein–protein interaction (PPI) shows M interactors are associated with cardiac hypertrophy, mitochondrial dysfunction and cardiac arrhythmia. K Protein–protein interaction (PPI) reveals the ATPase subunits ATP5A1 and ATP5B are shared interactors by Nsp6/Nsp8/M proteins in hESC-CMs. L Co-IP Western-blotting verification of the interactions of ATP5A1/ATP5B with Nsp6/Nsp8/M proteins in hESCs-CMs. M ATP level detection in hESC-CMs. All bars are shown as mean ± SD. (n = 3). A two-tailed unpaired t test was used to calculate p values. *p < 0.05 (vs. Control). N ATP level detection in hiPSC-CMs. All bars are shown as mean ± SD. (n = 3). A two-tailed unpaired t test was used to calculate p values. *p < 0.05 (vs. Control)