Fig. 6
FDA-approved drugs can ameliorate SARS-CoV-2 genes-induced CM abnormalities. A ATP levels in hESC-derived CMs. The final concentration of ivermectin and meclizine's is 0.5 µM. Treatment time is 3 h. All bars are shown as mean ± SD. (n = 3). A two-tailed unpaired t test was used to calculate p values. *p < 0.05, **p < 0.01, ***p < 0.001. Black * indicates overexpression versus control; yellow * indicates ivermectin treatment versus no drug; purple * indicates meclizine versus no drug. B–E Representative immunostaining images of TUNEL+ and CTNT+ CMs in control hiPSC-EBs (B), Nsp6OE hiPSC-EBs (C), Nsp8OE hiPSC-EBs (D) and MOE hiPSC-EBs (E). The final concentration of ivermectin and meclizine's is 0.5 µM. Treatment time is 48 h. Scale bar, 100 µm. F Statistical data analysis of immunostaining results from B–E. All bars are shown as mean ± SD (n = 3). A two-tailed unpaired t test was used to calculate p values. *p < 0.05, **p < 0.01, ***p < 0.001. Black * indicates overexpression versus control; yellow * indicates ivermectin treatment versus no drug; purple * indicates meclizine versus no drug. G Calcium imaging results in control hESC-CMs. Y-axis represents intensity and X-axis represents time (in seconds, s). H Calcium imaging results in Nsp6OE, Nsp8OE and MOE hESC-CMs treated without or with drugs for 1 h. Y-axis means intensity. X-axis represents time (in seconds, s). I Interspike interval (ISI) distribution analysis from G. Y-axis means beating frequency in a specific time window. X-axis means time (in seconds, s). J Interspike interval (ISI) distribution analysis from (H) in different CM cell lines treated with or without drugs for 1 h