Fig. 1

Injury-experienced SCs proliferate sooner and form bigger myotubes in vitro: A schematic diagram of the experimental approach. B Representative images of Pax7⁺/ki-67⁺ SCs, isolated from uninjured muscle or 50 days after cardiotoxin injury (50 DPI) and cultured for 48 h. C Quantification of Pax7⁺/ki-67⁺ SCs as in B. D Representative images of MHC⁺ myotubes formed by SCs isolated 50 DPI or control and cultured in growth medium for 72 h followed by additional 24 h in differentiation medium. E Quantification of the percentage of myotubes with 2 or less, 3 to 5, or more than 5 nuclei. H Quantification of myotube size, expressed as medium diameter in microns. n = 3 independent samples. Data are shown as mean ± S.E.M. *p < 0.05; **p < 0.01