Fig. 5

Differential cytokines expression in muscle upon repeated cardiotoxin injury: A–D Quantification of CCL-2 (A), IL-1β (B), IL-6 (C) and IL10 (D) relative expression as measured by RT-qPCR. RNA was extracted from mononuclear cells isolated from muscle at 18 h, 3 days, 10 days and 30 days after single or repeated cardiotoxin injury. Data are expressed as fold change (2-ddCT) normalized against GAPDH. Data are shown as mean ± S.E.M. *p < 0.05. E UMAP plot of whole-muscle single-cell RNAseq database from Oprescu et al. [26]. F Single-cell expression patterns of CCL2, IL-1β, IL-6 and IL10 in injured (2 or 10 days post-cardiotoxin) or uninjured control (from Oprescu et al. [26])