Fig. 4
From: Inflammation-inducible promoters to overexpress immune inhibitory factors by MSCs
The influence of CXCL11S1-driven IL10 overexpression by UC-MSCs in an ALI mouse model. Human UC-MSCs were transduced with a lentiviral vector coding for a constitutively expressed mCherry driven by a PGK promoter as transduction control. Additionally, the vector coded for eGFP or human IL10 under the control of CXCL11S1 promoter. C57BL/6 J mice were intratracheally treated with 50 µL of 5 µg or 10 µg LPS to induce ALI. After 4 h, PBS or 5 × 105 MSCs either transduced with CXCL11S1-driven eGFP or IL10 were injected into the tail vein. Mice were killed after an additional 24 h, the bronchoalveolar lavage fluid (BALF) was collected, lungs were digested and the immune composition analyzed by flow cytometry analyzed. A Mouse weight loss during experiment. B Number of cells in the BALF. C Tnf-α concentration in the first 1.5 mL of the BALF quantified with ELISA. D Frequency of Cd19 + cells in Cd45 + cells. E Frequency of Cd3 + Cd8 + cells in Cd45 + cells. F Frequency of Cd3 + Cd4 + cells in Cd45 + cells. G Frequency of Cd11b + Cd11c + cells in Cd45 + cells. H Frequency of Cd11b + Ly6g + cells in Cd45 + cells. Depicted are the means. Errors bars indicate SD. Statistical significance was calculated with Two-way-ANOVA and subsequent Bonferroni-corrected pairwise t-test. *: p < 0.05, **: p < 0.01, ***: p < 0.001