Fig. 1

ADPRHL1 deficiency does not affect CM differentiation, but disrupts CM adhesion. A Structure of the ADPRHL1 locus showing localization of gRNA for epiCRISPR/Cas9 editing. B) Sequence chromatograms demonstrate 5-base insertion in ADPRHL1 knockout (KO) colonies. C Immunofluorescence staining of KO colonies for the pluripotency markers SOX2 and SSEA4. Scale bar, 10 μm. D and E Flow cytometry analysis for TNNT2 from representative WT and KO differentiation protocols before purification at day 15. F Western blot analysis of ADPRHL1 in WT-CMs and KO-CMs at day 15; G Quantification of ADPRHL1 normalized by GAPDH in WT-CMs and KO-CMs. H Adherence of WT-CMs and KO-CMs after re-seeding on day 15. Scale bar, 50 μm. The results are presented as means ± SEMs of 3 independent experiments. ns, not significant, unpaired 2-sided Student’s t test